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Workshops – details
Workshop 1 – the tissue-specific proteome
The specific proteins expressed in various human cell types, tissues and organs constitute important targets for organ-specific research in health and disease, and are interesting to study in order to understand their functional role with respect to phenotypic expression in human biology. In this workshop, we will explore the proteins enriched in various tissue and cell types in the human body. A comprehensive in-depth classification of these proteins and their exact cell-type specific localization within the tissue will be performed for selected tissues in order to further explore and elucidate their functional characteristics. After the workshop, the participants will have gained insights into the proteins expressed in a tissue-specific manner based on data from several assay platforms, including antibody-based methods and transcriptomics.
Workshop 2 – the missing proteome
The human genome sequence has identified approximately 20,000 genes coding for proteins, that if studied in-depth, will provide knowledge crucial for understanding the human biology in a holistic manner. The majority of these genes and their corresponding proteins are well known but large knowledge gaps still remain. More than 1,900 genes express proteins with still unknown functions. An essential part of bridging these gaps is to define the localization of these proteins across the human cells and to annotate the functional characteristics of each protein. In this workshop, we will focus on the proteins with no or little previous information on the protein level. Experimental results from antibody and mass spectrometry based experiments, and RNA-sequencing will be assessed to find evidence for the existence of these proteins. The workshop will provide participants with knowledge on how to extract and interpret experimental results for functional annotation of unknown proteins.
Workshop 3 – the human plasma proteome
The presence of various protein levels in blood is an indispensable tool that helps us to understand and explain human biology at a systems level. Blood has always played a crucial part in clinical diagnostics thanks to its accessibility and relatively non-invasive sampling method. The blood proteins and our ability to quantify them are crucial for precision medicine efforts and future attempts to discover new biomarkers when monitoring health and detecting early signs of disease. This workshop will take a deeper look into the secreted protein repertoire using the Blood Atlas as well as all other proteins found in the blood circulation. The participants will discuss and actively work on annotating the different blood proteins in terms of their classes, origin, cellular location, and methods used for these analyses. After the workshop, the participants will have gained new insights about the Plasma Proteome, understanding its composition and complexity as well as the analytical challenges that researchers face when using the circulating proteins in precision medicine.
Workshop 4 – the essential proteome
The definition of a minimal set of proteins needed to maintain the basal functionality of any given cell (the essential proteome) is an important starting point for better understanding of the core machinery in the cell and the difference between various cell types. The essential proteome includes the proteins needed for translation, transcription, metabolism and maintenance of essential structural features for both dividing and non-dividing cells. In this workshop, we will explore the features of and the evidence for essential genes by combining data from genome-wide knock-out studies, transcriptomics and antibody-body based analyses with functional data. The participants will discuss different aspects and definitions of an essential protein, and by exploring expression patterns, functions and other features of the resulting sets of proteins make an attempt to define the “essential proteome”. After this workshop the participants will have gained insights about different aspects of essential genes and how to extract and analyze data from various databases to explore their features. They will further get an understanding of some of the complexity and challenges of protein categorization and data evaluation.